Role of Catalase Enzyme in the Existence of Allicin on Hep-G2 Cell Line
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Abstract
In recent years, there has been significant attention on the use of chemical and bioactive compounds extracted from medicinal plant sources to prevent and treat various diseases. Among the active components of herbal medicines, curcumin, resveratrol, quercetin, emodin, silybin, and their derivatives have been particularly discussed in the treatment of chronic and common disorders such as cardiovascular, metabolic, neurogenic diseases, and cancer. It is estimated that about sixty percent of currently widely used anticancer drugs are derived from natural sources. These compounds also play an important role in cancer prevention, due to their high ability to scavenge free radicals, inhibit cell growth, and induce apoptosis. For centuries, garlic and its derived compounds have attracted researchers' attention due to their health-promoting effects. Various bioactive compounds in garlic are responsible for its health benefits, among which allicin is one of the key components. Allicin is responsible for the pungent odor of garlic and is one of the most important organosulfur compounds in garlic, making up about 70% of the thiosulfate compounds. Allicin is produced during the crushing of garlic through the enzymatic activity of alliinase on alliin and is effective against a wide range of microorganisms. The antimicrobial and antioxidant properties of allicin are attributed to its reaction with the thiol groups of various enzymes. Allicin prevents cancer onset and progression by blocking metastasis and inhibiting excessive cell proliferation. This study investigates the role of the enzyme catalase in the presence of allicin on the Hep-G2 cell line. Allicin, the active ingredient in garlic, was applied in its pure form to Hep-G2 cancer cells in a concentration range from 0 to 250 µg/mL for 24 hours. The MTT assay revealed that allicin had an inhibitory effect on the proliferation of these cells. A proposed mechanism for the cytotoxic effect of allicin is the impact of oxygen free radicals on the biochemical structure of these cells. The general reduction in catalase enzyme activity leads to an increase in these free radicals. Ultimately, by inducing oxidative stress and decreasing catalase activity, allicin causes cellular toxicity and exhibits anticancer properties on Hep-G2 cells.
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References
Nirmala MJ, Samundeeswari A, and S. PD, Natural plant resources in anti-cancer therapy-A review. Res Plant Biol, 2011. 1(3): p. 01-14.
Justin B. Hellmana , G.J.H., Lily Koo Lina, , Successful treatment of metastatic congenital intraocular medulloepithelioma with neoadjuvant chemotherapy, enucleation and superficial parotidectomy, . American Journal of Ophthalmology Case Reports 11, 2018: p. 124–12.
Das, S., S. Mukherjee, and D. Vasudevan, Medicinal properties of milk thistle with special reference to silymarin– An overview. Natural Product Radiance, 2008. 7: p. 182-192.
Loguercio C, F.D., Silybin and the liver: from basic research to clinical practice. World journal of gastroenterology, 2011. 17(18): p. 2288-301.
Rezakhani Laila, M.P., Alizada Akram, Khazaai Mohammad Rasool, Alizada Murtaza,, An Overview of plants and Natural Products with Anti-Cancer Effects,. 21, 2018. 3: p. 163-171.
Lu Q, L.P.-M., Piao J-H, Xu X-L, Chen J, Zhu L, et al. , Preparation and physicochemical characteristics of an allicin nanoliposome and its release behavior. LWT-Food science and technology. LWT-Food science and technology, 2014. 57(2): p. 686-95.
Mohammad Ali Fakhiri, S.A.S.A., Sana Akaber, Investigating the properties, structure and role of the bioactive compound allicin and allinase enzyme in the occurrence of medicinal nutritional properties of garlic. 14th International Conference on Food Industry Sciences, Organic Farming and Food Security, 2023.
S, N., Antioxidants and their role in biological functions: An overview. Indian Drugs, 2003. 40(9): p. 501-16.
Ehsana tahiri, et al., Comparing the activity of antioxidant enzymes superoxide dismutase, glutathione peroxidase and glutathione reductase in type 2 diabetic red blood cells compared to healthy people. 2012. 5: p. 464–473
Marjan qurbani anarkuli, S.d., Hasan mawladost, Adib zendadol, Mohammad hadifahadri, Comparison of MTT, trypan blue and clonogenic assay methods in determining cell line survival Human anaplastic thyroid carcinoma. 2019. 1(7): p. 22-26.
Christophe Glorieux1 and P.B. Calderon, Catalase down-regulation in cancer cells exposed to arsenic trioxide is involved in their increased sensitivity to a pro-oxidant treatment. Glorieux and Calderon Cancer Cell Int 2018. 18(24).
Ludlow, R.A., Pacenza, M., Chiappetta, A., Christofides, S. R., Evans, G., Graz, M., ... & Müller, C. T., Storage time and temperature affects volatile organic compound profile, alliinase activity and postharvest quality of garlic. . Postharvest Biology and Technology, 2021. 177.
Wolf Bat-Chen, Tal Golan, Irena Peri, Zvi Ludmer & Betty Schwartz, Allicin Purified From Fresh Garlic Cloves Induces Apoptosis in Colon Cancer Cells Via 2010. 62(7): p. 947-957.
John D. Hayes, Albena T. Dinkova-Kostova,1,2 and Kenneth D. , Tew3, Oxidative Stress in Cancer .
Asaduzzaman Khan, Md., M.T., Dian-zheng Zhang, Han-chun Chen, , Antioxidant Enzymes and Cancer, . Chin J Cancer Res 2010. 22(2): p. 87-92.
Elchuri S, O.T., Qi W, Eisenstein RS, Jackson RL, Van-Remmen H, Epstein CJ andHuangTT. CuZnSOD deficiency leads to persistent and widespread oxidative damage andhepatocarcinogenesis later in life. Oncogene. 2005. 24(3): p. 367-380.
Chaudhari, A., Cytotoxic Potential of Allicin in the Inhibition of Different Human Breast Cancer Cell Lines, . 2022.
Christophe Glorieux, J.M.S., Nicolas Dejeans, Sandrine Nonckreman, Khadija Bahloula, Hélène A. Poirel , and Pedro Buc Calderon, , Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells,. 2018: p. 10.